However, in the present study, there was no difference between vitrification and slow freezing techniques on the cryopreservation of zebrafish ovarian tissue containing immature follicles.
In the second part of the work, different protocols of sperm cryopreservation were compared, namely conventional slow freezing with cryoprotectant (SFCPA); slow freezing with seminal plasma (SFSEM); direct vitrification in a cryovial with CPA (VITCPA); direct vitrification in a cryovial with seminal plasma (VITSEM); vitrification using a new vitrification device in a cryovial with CPA …
Vitrification is a modern technique that rapidly freezes reproductive cells to a temperature of -196°C, literally within seconds.
Slow cooling Prior to slow cooling of both oocytes and embryos, dehydration without excessive shrinkage is achieved by exposure to permeating cryoprotectants at concentrations ≤1.5 M and non-permeating cryoprotectants at … These principles are fundamental to cryopreservation but are applied in different ways in the methods used for slow cooling and vitrification in clinical ART. The major difference between them is the total avoidance of ice formation in vitrification. However, both slow freezing and vitrification resulted in a loss of embryo quality after thawing without statistical differences between the two cryopreservation procedures. Cryopreservation is a technique that utilises a special medium to allow preservation in liquid nitrogen at a temperature of -196°C.
There are two major techniques for cryopreservation: freeze-thaw processes and vitrification. The difference between cryopreservation and vitrification is in the cell freezing rate.
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